Product Details
Catalouge Number
– ACS 31505/0008
Description
– AmplifyRP®
Acceler8® for PPV (Plum pox virus)
Sizes
– 8T,
Applications
– AmplifyRP-Acceler8
Applications Notes
–
Test Label:
Biotin labeled primer / Fam labeled probe
Test Format: AmplifyRP® Acceler8®
Test Format: AmplifyRP® Acceler8®
Kit Components
–
Acceler8®
reaction pellets (8)
Amplicon detection chambers (8)
PD1 Pellet diluent (1.0 ml)
GEB4 sample extraction bags (8)
1µl transfer loops (10)
User Guide
Amplicon detection chambers (8)
PD1 Pellet diluent (1.0 ml)
GEB4 sample extraction bags (8)
1µl transfer loops (10)
User Guide
Features & Benefits
–
AmplifyRP®
Acceler8® for PPV is a rapid RNA amplification and detection platform
designed for field-based or laboratory testing of prunus crops for Plum pox
virus.
Plum pox virus (Sharka disease) is an aphid transmitted potyvirus that can cause
significant loss of yield and marketability of stone fruit (peaches, plum,
cherry, etc). PPV is found around the world and is of particular importance
in Europe and other Mediterranean regions. PPV detections have been confirmed
in the U.S. and Canada in the late 1990’s and early 2000’s which instigated
large scale testing and eradication programs due to the severity of the
disease.
AmplifyRP® Acceler8® enables users to rapidly amplify and test for PPV RNA
at a single operating temperature using only a crude sample extract.
Amplified product is then tested in an Amplicon Detection Chamber where clear
“yes / no” results are read visually on a lateral flow strip (much
like a pregnancy test). The entire testing process can be completed in as
little as 30 minutes by users of any skill level.
AmplifyRP® Acceler8® for PPV was designed to run in GEB4 extraction buffer;
the same extraction buffer used in Agdia’s PPV ELISA (USDA approved screening
method for PPV) as well as the ImmunoStrip®. This allows molecular
confirmation of ELISA or ImmunoStrip® results from the exact same plant
extract.
AmplifyRP® Acceler8® for PPV detects ALL known strains of the virus
including PPV-An, PPV-C, PPV-CR, PPV-D, PPV-EA, PPV-M, PPV-Rec, PPV-T, and
PPV-W.
Product Attachments
Data Sheets
Safety Data Sheets
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