– MAB238 characterization from Brandon et al. (2): Mab 238 was made against classical Bowman-Birk trypsin/chymotrypsin inhibitor from Glycine max. Isotyping was done using reagents from SouthernBiotechnology (Birmingham, AL), and was determined to be IgG¹. The Bowman-Birk inhibitor supernatant reactivity has been studied in Glycine max tissue only. This monoclonal antibody is not known to reliably react with Bowman-Birk type inhibitors from other species (1). Antigen Binding Region: According to Kollipara et al., the antigen binding region of MAB 238 was determined to be around the trypsin-reactive domain rather than the chymotrypsin-reactive domain. Cell culture Supernatant: Antibody yield per ml was determined to be 1.0mg/ml when purified by ammonium sulfate. Sodium Azide was added as the preservative at 0.05%. Storage: Store the material at 4°C for short term storage. When storing long term, keep at -20°C. Avoid freeze thaw cycles.